Part:BBa_M36142:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M36142
User Reviews
UNIQa8f8d0318ca5905e-partinfo-00000000-QINU UNIQa8f8d0318ca5905e-partinfo-00000001-QINU 12/04/15
Mature EP-B2 was used in comparison to Pro EP-B2 to test whether the inhibitor sequence of the Pro EP-B2 was necessary for the protein to be made. Our fluorescence assay showed that mature EP-B2 fluoresced sufficiently meaning that the inhibitor sequence was not necessary. A fluorescence assay was performed to test presence of Mature EP-B2 in SDS lysed E.Coli. The fluorescence assay showed that Mature EP-B2 was found to be in cells, since the reading detected increasing presence of 5-FAM quencher in the samples, a quencher that is cleaved by the Mature EP-B2 protein. Similar results were also found for the Mature EP-B2. The Mature EP-B2 actually was expressed at much higher RLU than the ProEP-B2, so it is possible that the inhibitor was responsible for the smaller expression found in ProEP-B2 compared to MatureEP-B2 (graphs shown below for comparison). More testing is needed to conclude that the inhibitor was responsible for the difference in expression.